U-558

Material Data Sheet

Ubiquitin-Lys-TAMRA (Ub-Gly-Gly-ε-Lys-TAMRA-Gly-OH)

Price: $225.00
Catalog #: U-558

Ubiquitin-Lys-TAMRA (Ub-Gly-Gly-ε-Lys-TAMRA-Gly-OH)

Price: 
$225
Quantity: 
50 µg
Data Sheet: 

Fluorescence polarization high-throughput screening (HTS) reagent which is based on a 5-tetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain. This reagent is useful for studying ubiquitin C-terminal hydrolytic activity when detection sensitivity or continuous monitoring of activity at longer wavelengths is essential.

Product Information

Stock: 
 X mg/ml (XμM) in DMSO.Concentration varies with lot number.
MW: 
9.2 kDa
Purity: 
> 95% by HPLC

Use & Storage

Use: 

Typical substrate concentrations range from 10-50nM and typical enzyme concentrations range from 0.5-10 nM but depend on specific assay conditions and method of detection. Recommended assay buffer is 50 mM Hepes pH 7.5, 150 mM NaCl, 2 mM DTT. Fluorescence can be monitored using Ex544 nm and Em572 nm wavelengths.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hassipen U., et al. (2007) Anal. Biochem. 371:201-207

Lavis L.D., et al. (2006) ACS Chemical Biology 4 : 252-260

Tirat A., et al. (2005) Anal. Biochem. 343:244-255