U-556

Material Data Sheet

Ubiquitin-Aminoluciferin (AML)

Price: $215.00
Catalog #: U-556

Ubiquitin-Aminoluciferin (AML)

Price: 
$215
Quantity: 
50 µg

Substrate for deubiquitinating enzymes based on the C-terminal derivative of ubiquitin with aminoluciferin (AML). Rather than fluorescence as the indicator of DUB activity, DUB liberated luciferin is processed by luciferase to give a luminescence signal. Ub-AML not only produces a stronger signal, but also has an excellent signal to noise ratio over traditional fluorophores. This makes it possible to rapidly assess the activity of DUBs that poorly utilize Ub-AMC while using much lower levels of the DUBs themselves.

Product Information

Stock: 
X mg/ml (X μM) in 50mM HEPES pH 7.5. Concentration varies with lot number.
MW: 
8.8 kDa
Purity: 
>95%
Activity: 

Over 100:1 signal to noise ratio with 10 pM UCH-L3.

Use & Storage

Use: 

Ub-AML gives a strong luminescent signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal luminescence at pH 7.5 is monitored using all wavelengths with a 500 ms integration time.

Storage: 
Store at -80°C. Avoid multiple freeze/ thaw cycles.

Literature

References: 

 

Reddy G., et al. (2010) JACS. 132:39, 13586-13587

Shinde R., et al. (2006) Biochemistry 45 : 11103-11112