U-555

Material Data Sheet

Ubiquitin-Rhodamine 110 (Rh110)

Price: $269.00
Catalog #: U-555

Ubiquitin-Rhodamine 110 (Rh110)

Price: 
$269
Quantity: 
50 µg
Data Sheet: 

Also known as: Ub-R110

Fluorogenic substrate for deubiquitinating enzymes based on the C-terminal derivative of ubiquitin with Rhodamine110 (R110). Similar to other C-terminus derivatives such as Ub-AMC (U-550) and Ub-AFC (U-551), this is an exquisitely sensitive deubiquitinating enzyme substrate and is useful for studying ubiquitin C-terminal hydrolytic activity when detection sensitivity or continuous monitoring of activity at longer wavelengths is essential.

Product Information

Stock: 
2.25 mg/ml (250 μM) in DMSO
MW: 
8.9 kDa
Purity: 
> 95%
Activity: 

UCH-L3 Km = 0.034 μM, USP2CD Km = 1.5 μM.

Use & Storage

Use: 

Ubiquitin-­Rhodamine110 (R110) is ideal for use in assays requiring fluorescent detection. Optimal fluorescence at pH 8.0 is monitored with an excitation wavelength of 485 nm and an emission wavelength of 535 nm.  Reaction conditions will need to be optimized for each specific application. We recommend an initial Ubiquitin­R110 concentration of 0.1-­1 μM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.