U-555

Material Data Sheet

Ubiquitin-Rhodamine 110 (R110)

Price: $250.00
Catalog #: U-555

Ubiquitin-Rhodamine 110 (R110)

Price: 
250
Quantity: 
50 µg
Data Sheet: 
application/pdf iconU-555 UbR110.pdf

Also known as: Ub-R110

Fluorogenic substrate for deubiquitinating enzymes based on the C-terminal derivative of ubiquitin with Rhodamine110 (R110). Similar to other C-terminus derivatives such as Ub-AMC (U-550) and Ub-AFC (U-551), this is an exquisitely sensitive deubiquitinating enzyme substrate and is useful for studying ubiquitin C-terminal hydrolytic activity when detection sensitivity or continuous monitoring of activity at longer wavelengths is essential.

Product Information

Stock: 
X mg/ml (XμM) in DMSO. Concentration varies with lot number.
MW: 
8.9 kDa
Purity: 
> 95%
Activity: 

UCH-L3 Km = 0.034 μM, USP2CD Km = 1.5 μM.

Use & Storage

Use: 

μM,Ub-R110 gives a strong signal in the range of 0.1-1 depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex485 nm and Em535 nm wavelengths respectively.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hassipen U., et al. (2007) Anal.Biochem. 371:201-207
Lavis L.D., et al. (2006) ACS Chemical Biology 4 : 252-260
Tirat A., et al. (2005) Anal.Biochem. 343:244-255