U-551

Material Data Sheet

Ubiquitin-AFC

Price: $159.00
Catalog #: U-551

Ubiquitin-AFC

Price: 
$159
Quantity: 
50 µg
Data Sheet: 

Fluorogenic substrate based on the C-terminus derivatization of Ubiquitin with 7-amino-4-trifluoromethylcoumarin (AFC).  Similar to ubiquitin-AMC, this is an exquisitely sensitive deubiquitinating enzyme substrate and is useful for studying Ubiquitin C-terminal hydrolytic activity when detection sensitivity or continuous monitoring is essential. The fluorophore has a larger Stokes shift than AMC which is useful to reduce compound interference in HTS assays.

Product Information

Stock: 
X mg/ml (X μM) in 100 % DMSO.
MW: 
8.6 kDa
Purity: 
> 95% by HPLC

Use & Storage

Use: 

Substrate concentrations for assay range from 0.1-1 μM.  Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AFC fluorescence by UCH enzymes can be monitored using Excitation and Emission wavelengths of 380 nm and 505 nm, respectively. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.solubilization. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dang L.C., et al. (1998) Biochem. 37:1868-1879
Gossrau R., et al (1984) Ad.Exp.Med.Biol. 167:191-207
Lojda Z (1996) Ad. Exp. Med.Biol. 98:215-228
Mason D.E., et al. (2004) Biochem. 43:6535-6544
Sinha P., et al. (1984) Ad. Exp.Med.Biol. 167:219-226
Smith R.E., et al. (1980) Throm.Res. 17:393-402
Stein R.L., et al. (1998) Biochem. 34:12616-12623