U-550

Material Data Sheet

Ubiquitin-AMC

Price: $229.00
Catalog #: U-550

Ubiquitin-AMC

Price: 
$229
Quantity: 
50 µg
Data Sheet: 

Fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Ubiquitin-AMC is an exquisitely sensitive substrate for UCH-L3 (Km = 0.039 μM) and for the Isopeptidase-T (Km = 0.17 - 1.4 μM). Ub-AMC is useful for studying ubiquitin hydrolases when detection sensitivity or continuous monitoring of activity is essential.

Product Information

Stock: 
X mg/ml (X μM) in 100 % DMSO.
MW: 
8.6 kDa
Purity: 
> 95% by HPLC

Use & Storage

Use: 

Ubiquitin-­AMC is a fluorogenic substrate for some Ubiquitin­specific isopeptidases.  Release of AMC fluorescence can be monitored with an excitation wavelength of 345 nm and an emission wavelength of 445 nm.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial Ubiquitin­AMC concentration of 0.1-­1 μM

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.solubilization. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dang L.C., et al. (1998) Biochem. 37:1868-1879
Mason D.E., et al. (2004) Biochem. 43:6535-6544
Stein R.L., et al. (1998) Biochem. 34:12616-12623