U-550

Material Data Sheet

Ubiquitin-AMC

Price: $200.00
Catalog #: U-550

Ubiquitin-AMC

Price: 
$200
Quantity: 
50 μg
Data Sheet: 
application/pdf iconU550_UbAMC.pdf
Stock: 

X mg/ml (X μM) in 100 % DMSO Concentration varies with lot number.

MW: 
8.6 kDa
Purity: 
> 95% by HPLC

Fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Ubiquitin-AMC is an exquisitely sensitive substrate for UCH-L3 (Km = 0.039 μM) and for the Isopeptidase-T (Km = 0.17 - 1.4 μM). Ub-AMC is useful for studying ubiquitin hydrolases when detection sensitivity or continuous monitoring of activity is essential.

Use & Storage

Use: 

Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.solubilization. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dang L.C., et al. (1998) Biochem. 37:1868-1879
Mason D.E., et al. (2004) Biochem. 43:6535-6544
Stein R.L., et al. (1998) Biochem. 34:12616-12623