U-540

Material Data Sheet

GST-Ubiquitin

Price: $105.00
Catalog #: U-540

GST-Ubiquitin

Price: 
$105
Quantity: 
1 mg
Data Sheet: 
application/pdf iconU540_gstUb.pdf
Stock: 
[GST-Ub] = X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.
MW: 
35 kDa
Purity: 
>95% by SDS-PAGE

Fully functional ubiquitin with an N-terminal GST-tag (glutathione S-transferase). GST-Ub allows for glutathione affinity purification of ubiquitinated molecules and allows for immuno- detection of conjugates using GST antibodies. Facilitates the facile visualization of poly-Ub chains due to larger ladder intervals.

Use & Storage

Use: 
Typical in vitro concentrations for non rate-limiting conjugate formation is 20-100 μM depending on conditions. Supplementation with ubiquitin may be necessary
for best results in forming high molecular weight conjugates. 
Storage: 
Store at -20°C after solubilization. Avoid multiple freeze/thaw cycles.

Literature

References: 

McTigue M.A., et al. (1995) J.Mol.Biol. 246:21-27

Scheffner M., et al. (1993) Cell. 75:495-505