U-530

Material Data Sheet

His6-Ubiquitin

Price: $85.00
Catalog #: U-530

His6-Ubiquitin

Price: 
$95
Quantity: 
2 mg
Data Sheet: 
application/pdf iconU530_hisUb.pdf
MW: 
8.5 kDa
Purity: 
>95% by SDS-PAGE
Solubility: 
Soluble in aqueous buffers up to 50 mg/ml.
Supplied: 

This is a lyophilized powder

Fully functional N-terminal His-tagged ubiquitin which allows for metal chelate affinity purification of ubiquitinated proteins. Also allows for convenient immuno-detection of conjugates using His6-specific antibodies.

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugation reactions are 200 μM-1 mM depending on reaction conditions.

Storage: 
Store at -20°C after solubilization. Avoid multiple freeze/thaw cycles.

Literature

References: 

Beers E.P. and Callis J. (1993) J.Biol.Chem. 268:21645-21649
Treier M., et al. (1994) Cell. 78:787-798