U-530

Material Data Sheet

His6-Ubiquitin

Price: $99.00
Catalog #: U-530

His6-Ubiquitin

Price: 
$99
Quantity: 
2 mg
Data Sheet: 

Recombinant N-terminal His6-tagged Ubiquitin

Product Information

Stock: 
2.4 mg/ml (250 μM) in 10 mM HEPES pH 7.5
MW: 
9.6 kDa
Purity: 
>95% by SDS-PAGE under reducting conditions and visualized by Colloidal Coomassie Blue Stain

Use & Storage

Use: 

Recombinant Human His6-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3).  Reaction conditions will need to be optimized for each specific application.  We recommend an initial His6-Ubiquitin concentration of 10-50 μM.

Storage: 

Store at -20°C or -80°C