U-530

Material Data Sheet

His6-Ubiquitin

Price: $99.00
Catalog #: U-530

His6-Ubiquitin

Price: 
$99
Quantity: 
2 mg
Data Sheet: 

Recombinant N-terminal His6-tagged Ubiquitin

Product Information

MW: 
9.6 kDa
Purity: 
>95% by SDS-PAGE
Supplied: 
Supplied as a lyophilized powder

Use & Storage

Use: 

Recombinant Human His6-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3).  Reaction conditions will need to be optimized for each specific application.  We recommend an initial His6-Ubiquitin concentration of 10-500 μM.

Storage: 

Store at -20°C after reconstitution in aqueous buffer

Literature

References: 

Beers E.P. and Callis J. (1993) J.Biol.Chem. 268:21645-21649
Treier M., et al. (1994) Cell. 78:787-798