U-501

Material Data Sheet

Methylated Ubiquitin

Price: $55.00
Catalog #: U-501

Methylated Ubiquitin

Price: 
$55 $225
Quantity: 
1 mg, 5 mg

Reductive methylation of lysine residues renders ubiquitin unable to form poly-ubiquitin chains. Methylated Ub can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (mono-ubiquitination). Ideal for studies in which a reduction in poly-ubiquitin chain length is desired, or in instances when multi-mono-ubiquitination of a substrate protein is needed.

Product Information

MW: 
8.5 kDa
Purity: 
>95% by SDS-PAGE
Solubility: 
Soluble in aqueous buffers up to 20 mg/ml.
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations for non rate-limiting support of in vitro conjugation reactions are 100 μM-500 µM depending on reaction conditions. 

Storage: 

Store at -20°C after solubilization. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hershko A. and Heller H. (1985) Bioch.Biophys.Res Comm. 128:1079-1086
Hershko A., et al. (1991) J.Biol.Chem. 266: 16376-16370
Jentoft N. and Dearborn D.G. (1979) J.Biol.Chem. 254:4359-4365
Jentoft N. and Dearborn D.G. (1980) Anal.Biochem. 106:186-190
Jentoft N. and Dearborn D.G. (1983) Methods.Enz. 91:570-579