Material Data Sheet
Quantity:1 mg, 5 mg
Reductive methylation of lysine residues renders ubiquitin unable to form poly-ubiquitin chains. Methylated Ub can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (mono-ubiquitination). Ideal for studies in which a reduction in poly-ubiquitin chain length is desired, or in instances when multi-mono-ubiquitination of a substrate protein is needed.
Purity:>95% by SDS-PAGE
Solubility:Soluble in aqueous buffers up to 50 mg/ml.
Supplied:This is a lyophilized powder
Use & Storage
Typical concentrations for non rate-limiting support of in vitro conjugation reactions are 100 μM-500 µM depending on reaction conditions.
Store at -20°C after solubilization. Avoid multiple freeze/thaw cycles.
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