U-400

Material Data Sheet

Ubiquitin Agarose

Price: $269.00
Catalog #: U-400

Ubiquitin Agarose

Price: 
$269 $159
Quantity: 
1 mL

Ubiquitin is covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for affinity binding of ubiquitin activating enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s), ubiquitin C-terminal hydrolases (UCHs), and other proteins/enzymes that have an affinity for ubiquitin.

Product Information

Stock: 
500 µl ubiquitin agarose is supplied as 1 ml of a 50% slurry in 20% ethanol.1 ml ubiquitin agarose is supplied as 2 ml of a 50% slurry in 20% ethanol.

Use & Storage

Use: 

Prepare the resin for use by washing with 5 ml buffered water to remove storage buffer. Equilibrate resin by washing with 5-10 ml desired start buffer. Typically, 100 mg Fraction II protein is applied to 1 ml Ub-agarose. Binding and elution of material is dependent on individual experimental conditions and requirements. Please refer to the supplied references for applicable protocols or contact us for specific application support.

Storage: 

Ub-agarose can be re-used for at least 10-15 applications if properly maintained. After use, clean resin by washing with 10 ml 50 mM Tris-Cl pH 9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM HEPES (or similar buffer) pH 7.5, containing either 1 mM NaN3 or 10-20 % ethanol. Resin should be stored at 4°C. DO NOT FREEZE

Literature

References: 

Ciechanover A., et al. (1982) J. Biol. Chem. 257:2537-2542
Hershko A., et al. (1983) J. Biol. Chem. 258:8206
Hersko A., et al. (1986) J.Biol. Chem. 261:11992.