U-214

New
Material Data Sheet

Ubiquitin-Propargylamide

Price: $199.00
Catalog #: U-214

Ubiquitin-Propargylamide

Price: 
$199
Quantity: 
50 µg
Data Sheet: 

Ubiquitin-Propargylamide (Ub-PA) is a potent, irreversible and specific inhibitor of C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs), and other deubiquitinases including the Otubains (OTU’s).  When mixed together, the reactive site cysteine of deubiquitinases and the    C-terminal alkyne functional group of Ub-PA rapidly form a covalent bond (vinyl thioether) that is resistant to denaturing conditions.  This product is useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.

Product Information

MW: 
8.6 kDa
Purity: 
> 95% by HPLC
Solubility: 
Soluble in DMSO up to 20 mg/ml
Activity: 

Ki =   < 40 pM versus UCHL3

Use & Storage

Use: 

Typical concentration to inhibit UCHs, USPs or DUBs in vitro is 0.5-5 µM.  It is highly recommended that lyophilized powder be reconstituted in DMSO, then further diluted in aqueous buffer to the desired final concentration.

Storage: 

Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ekkebus R., et al.  (2013) J. Am. Chem. Soc  135: 2867−2870

Sommer S., et al. (2013) Bioorg Med Chem.  21: 2511-7