U-212

Material Data Sheet

HA-Ubiquitin Vinyl Sulfone

Price: $235.00
Catalog #: U-212

HA-Ubiquitin Vinyl Sulfone

Price: 
$235
Quantity: 
25 µg
Data Sheet: 

Also known as: HA-Ub-VS

This N-terminal HA-tagged ubiquitin is a potent, irreversible and specific inhibitor of most deubiquitinating enzymes (DUBs) including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). Useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of DUBs since it is specifically recognized be by anti-HA antibodies and/or anti-HA-agarose.

Product Information

Stock: 
Supplied as a 2.5 mg/ml (250 µM) solution in 50 mM MES, pH 6.0
MW: 
9.9 kDa
Purity: 
> 95 % by HPLC.
Activity: 

Ki = <40 pM versus UCHL3

Use & Storage

Use: 

Depending on conditions typical concentrations to fully inhibit DUBs in vitro are 1-5 μM.  Also reacts with some E3 Ubiquitin Ligases such as Parkin.

Storage: 

Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196

Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159

Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291

Catic A., et al. (2007) PLoS One 2:e679

Dang L.C., et al. (1998) Biochem. 37:1868-1879

Galardy P., et al. (2005) Methods Enz. 399:120-131

Gredmark S., et al. (2007) J.Virol. 81:10300-10309

Hemelaar J., et al. (2004) Mol.Cel.Biol. 24:84-95

Hershko A. and Rose I.A. (1987) Proc.Natl.Acad.Sci. 84:1829-1833

Love K.R., et al. (2007) Nat.Chem.Biol. 3:697-705

Misaghi S., et all. (2005) J.Biol.Chem. 280: 1512-1520

Schieker C., et al. (2005) J.Virol. 79:15582-15585

Wang G., et al. (2003) Org.Lett. 5: 737-740

Verma R., et al. (2002) Science  298: 611-615