U-212

Material Data Sheet

HA-Ubiquitin-Vinyl Sulfone

Price: $235.00
Catalog #: U-212

HA-Ubiquitin-Vinyl Sulfone

Price: 
$235
Quantity: 
25 μg
Data Sheet: 

Also known as: HA-Ubiquitin Vinyl Sulfone (HA-Ub-VS)

This N-terminal HA-tagged ubiquitin is a potent, irreversible and specific inhibitor of most deubiquitinating enzymes (DUBs) including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). Useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation. The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of DUBs since it is specifically recognized be by anti-HA antibodies and/or anti-HA-agarose.

Product Information

MW: 
8.6 kDa
Purity: 
> 95 % by HPLC.
Solubility: 
Soluble in aqueous buffer up to 5 mg/ml. Pellet dry compound prior to reconstitution.
Activity: 

Ki = < 40 pM versus UCHL3

Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Depending on conditions typical concentrations to fully inhibit DUBs in vitro are 1-5 μM.

Storage: 

Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196
Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159
Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291
Catic A., et al. (2007) PLoS One 2:e679
Galardy P., et al. (2005) Methods Enz. 399:120-131
Gredmark S., et al. (2007) J.Virol. 81:10300-10309
Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95
Love K.R., et al. (2007) Nat.Chem.Biol. 3:697:705
Misaghi S., et al. (2005) J.Biol.Chem. 280: 1512-1520
Schieker C., et al. (2005) J. Virol. 79:15582-15585
Wang G., et al. (2003) Org.Lett. 5: 737-740
Verma R., et al. (2002) Science 298: 611-615