Also known as: HA-Ubiquitin Aldehyde (HA-Ub-H)

Ubiquitin-aldehyde is a potent and specific inhibitor of most deubiquitinating enzymes (DUBs) such as ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). It prevents the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation. This tagged version contains an N-terminal HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.