Material Data Sheet

HA-Ubiquitin Aldehyde

Price: $145.00
Catalog #: U-211

HA-Ubiquitin Aldehyde

50 µg
Data Sheet: 

Also known as: HA-Ubiquitin Aldehyde (HA-Ub-H)

Ubiquitin-aldehyde is a potent and specific inhibitor of most deubiquitinating enzymes (DUBs) such as ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). It prevents the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation. This tagged version contains an N-terminal HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.

Product Information

9.5 kDa
> 95% by HPLC.
Resuspend in aqueous buffer to 2 mg/ml. Pellet dry compound prior to reconstitution.

Ki = 2.5 nM vs. UCH-L3

This is a lyophilized powder

Use & Storage


Add Recombinant Human HA-Ubiquitin Aldehyde to in vitro assays to inhibit Deubiquitinating enzymes.  The HA-tag allows for detection and purification of Deubiquitinating enzymes activity.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial Recombinant Human HA-Ubiquitin Aldehyde concentration of 2-5 μM.

Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.



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