U-211

Material Data Sheet

HA-Ubiquitin Aldehyde

Price: $145.00
Catalog #: U-211

HA-Ubiquitin Aldehyde

Price: 
$145
Quantity: 
50 µg
Data Sheet: 

Also known as: HA-Ubiquitin Aldehyde (HA-Ub-H)

Ubiquitin-aldehyde is a potent and specific inhibitor of most deubiquitinating enzymes (DUBs) such as ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). It prevents the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation. This tagged version contains an N-terminal HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.

Product Information

MW: 
9.5 kDa
Purity: 
> 95% by HPLC.
Solubility: 
Resuspend in aqueous buffer to 2 mg/ml. Pellet dry compound prior to reconstitution.
Activity: 

Ki = 2.5 nM vs. UCH-L3

Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Add Recombinant Human HA-Ubiquitin Aldehyde to in vitro assays to inhibit Deubiquitinating enzymes.  The HA-tag allows for detection and purification of Deubiquitinating enzymes activity.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial Recombinant Human HA-Ubiquitin Aldehyde concentration of 2-5 μM.

Storage: 
Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196
Catic A., et al. (2007) PLoS One 2:679
Dang L.C., et al. (1998) Biochem. 37:1868-1879
Galardy P., et al. (2005) Methods Enz. 399:120-131
Gredmark S., et al. (2007) J.Virol. 81:10300-10309
Hemelaar J., et al. (2004) Mol.Cel.Biol. 24:84-95
Hershko A. and Rose I.A. (1987) Proc.Natl.Acad.Sci. 84:1829-1833
Love K.R., et al. (2007) Nat.Chem.Biol. 3:697-705
Melandri F.D., et al. (1996) Biochem. 35:12893-12900
Ovaa H., et al. (2005) Methods Enz. 399:468-478