U-203

Material Data Sheet

Ubiquitin-Vinyl Methyl Ester

Price: $269.00
Catalog #: U-203

Ubiquitin-Vinyl Methyl Ester

Price: 
$269
Quantity: 
50 µg
Data Sheet: 

A potent, irreversible and specific inhibitor of ubiquitin C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs) and deubiquitinating enzymes (DUBs). Useful for inhibiting the hydrolysis of polyubiquitin chains on substrate proteins in vitro and thus enhances polyubiquitin chain accumulation.

Product Information

MW: 
8.6 kDa
Purity: 
> 95 % by HPLC.
Solubility: 
Soluble in aqueous buffer up to 5 mg/ml. For best results, pellet dry compound prior to reconstitution.
Activity: 

Ki = < 40 pM versus UCHL3

Supplied: 
lyophilized powder

Use & Storage

Use: 

 Add directly to in vitro assay from the stock solution. Depending onconditions typical concentrations to fully inhibit UCHs, USPs or DUBsin vitro are 1-5 μM.

Storage: 

Store powder at -20°C. Store stock solution at -80°C.Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196

Borodovsky A., et al. (2002) Chem. Biol. 9: 1149-1159

Borodovsky A., et al. (2005) Chem. Biochem. 6: 287-291

Hemelaar J., et al. (2004) Mol. Cell. Bio. 24: 84-95

Misaghi S., et al. (2005) J. Biol. Chem. 280: 1512-1520

Wang G., et al. (2003) Org. Lett. 5: 737-740

Verma R., et al. (2002) Science. 298: 611-615