U-203

Material Data Sheet

Ubiquitin-Vinyl Methyl Ester

Price: $250.00

Catalog #: U-203

Ubiquitin-Vinyl Methyl Ester

Price:

250

Quantity:

50 µg

Data Sheet:

u-203-ubiquitin-vinyl-methyl-ester.pdf

A potent, irreversible and specific inhibitor of ubiquitin C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs) and deubiquitinating enzymes (DUBs). Useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.

Product Information

MW:

8.6 kDa

Purity:

> 95 % by HPLC.

Solubility:

Soluble in aqueous buffer up to 5 mg/ml. For best results, pellet dry compound prior to reconstitution.

Activity:

Ki = < 40 pM versus UCHL3

Supplied:

lyophilized powder

Use & Storage

Use:

Add directly to in vitro assay from the stock solution. Depending onconditions typical concentrations to fully inhibit UCHs, USPs or DUBsin vitro are 1-5 μM.

Storage:

Store powder at -20°C. Store stock solution at -80°C.Avoid multiple freeze/thaw cycles.