U-202

Material Data Sheet

Ubiquitin-Vinyl Sulfone

Price: $269.00
Catalog #: U-202

Ubiquitin-Vinyl Sulfone

Price: 
$269
Quantity: 
50 μg
Data Sheet: 

Also known as: Ub-VS

A potent and highly specific inhibitor of all ubiquitin C-terminal hydrolases. This protein blocks the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.

Product Information

Stock: 
Supplied as a 2.15 mg/ml (250 µM) solution in 50 mM MES pH 6.0
MW: 
8.6 kDa
Purity: 
> 98 % by HPLC.
Activity: 

Ki = < 40 pM versus UCHL3 

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions typical concentrations to fully inhibit UCHs, USPs or DUBs in vitro are 1-5 μM. 

Storage: 

Store stock solution at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196
Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159
Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291
Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95
Misaghi S., et all. (2005) J.Biol.Chem. 280: 1512-1520
Wang G., et al. (2003) Org.Lett. 5: 737-740
Verma R., et al. (2002) Science. 298: 611-615