Ubiquitin Vinyl Sulfone
Catalog #: U-202
Product Source:
human recombinant
Also known as: Ub-VS
A potent and highly specific inhibitor of all ubiquitin C-terminal hydrolases. This protein blocks the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.
Product Information
Solubility:
Soluble in aqueous buffer up to 5 mg/ml. For best results, pellet dry compound prior to reconstitution.
Activity:
Ki = < 40 pM versus UCHL3
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Add directly to in vitro assay from the stock solution. Depending on conditions typical concentrations to fully inhibit UCHs, USPs or DUBs in vitro are 1-5 μM.
Storage:
Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Literature
References:
Borodovsky A., et al . (2001) EMBO J. 20 : 5187-5196et al . (2002) Chem.Biol . 9 : 1149-1159et al . (2005) Chem.Biochem . 6 : 287-291et al . (2004) Mol.Cell.Bio . 24 : 84-95et al l. (2005) J.Biol.Chem . 280 : 1512-1520et al . (2003) Org.Lett. 5 : 737-740et al . (2002) Science . 298 : 611-615
