U-202

Material Data Sheet

Ubiquitin-Vinyl Sulfone

Price: $250.00
Catalog #: U-202

Ubiquitin-Vinyl Sulfone

Price: 
$250
Quantity: 
50 μg
Data Sheet: 
application/pdf iconU-202_Ub VS.pdf
MW: 
8.6 kDa
Purity: 
> 98 % by HPLC.
Solubility: 
Soluble in aqueous buffer up to 5 mg/ml. For best results, pellet dry compound prior to reconstitution.
Activity: 
Ki = < 40 pM versus UCHL3
Supplied: 
This is a lyophilized powder

Also known as: (Ub-VS)

A potent, irreversible and specific inhibitor of all ubiquitin C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs) and deubiquitinating enzymes (DUBs). Useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions typical concentrations to fully inhibit UCHs, USPs or DUBs in vitro are 1-5 μM.

Storage: 
Store powder at -20°C. Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196

Borodovsky A., et al. (2002) Chem.Biol. 9: 1149-1159

Borodovsky A., et al. (2005) Chem.Biochem. 6: 287-291

Hemelaar J., et al. (2004) Mol.Cell.Bio. 24: 84-95

Misaghi S., et al. (2005) J.Biol.Chem. 280: 1512-1520

Wang G., et al. (2003) Org.Lett. 5: 737-740

Verma R., et al. (2002) Science. 298: 611-615