U-201

Material Data Sheet

Ubiquitin Aldehyde (Ub-H)

Price: $129.00
Catalog #: U-201
Ubiquitin Aldehyde (Ub-H)
Price: 
$129
Quantity: 
50 μg
Data Sheet: 

Ubiquitin Aldehyde is a potent and specific inhibitor of Ubiquitin C-terminal hydrolases (UCHs), Ubiquitin-specific proteases (USPs) and Deubiquinating enzymes (DUBs). This protein blocks the hydrolysis of poly-Ubiquitin chains on substrate proteins in vitro and thus enhances poly-Ubiquitin chain accumulation.  Some DUBs, such as those of the JAMM class, are not inhibited by Ubiquitin Aldehyde.

Product Information

Stock: 
X mg/ml (X μM) in 50mM HCl
MW: 
8.5 kDa
Activity: 
Ki = 2.5 nM vs. UCH Isopeptidase-T.

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit UCHs in vitro are 2-5 µM.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles. Avoid presence of amino-containing compounds. Do not neutralize until immediately prior to use.
Purity: 
> 96% by HPLC.

Literature

References: 

Borodovsky A., et al. (2001) EMBO J. 20: 5187-5196

Burnett B., et al. (2003) Hum. Mol. Genet. 12: 3195-3205

Dang L.C., et al. (1998) Biochem. 37:1868-1879

Dunten R.L. and Cohen, R.E. (1989) J. Biol. Chem. 264:16739-16747

Johnston S.C., et al. (1999) EMBO J. 18: 3877-3887

Hershko A. and Rose I.A. (1987) Proc. Natl. Acad. Sci. 84:1829-1833

Hu M., et al. (2005) EMBO J. 2:

Melandri F.D., et al. (1996) Biochem. 35:12893-12900

Shaeffer J.R. and Cohen R.E. (1996) Biochem. 35:10886-10893