U-201

Material Data Sheet

Ubiquitin Aldehyde

Price: $110.00
Catalog #: U-201

Ubiquitin Aldehyde

Price: 
$110
Quantity: 
50 μg
Data Sheet: 
application/pdf iconU201_UbH.pdf
Stock: 

X mg/ml (XμM) in 50mM HCl

MW: 
8.5 kDa
Purity: 
> 96% by HPLC.
Activity: 

Ki = 2.5 nM vs. UCH Isopeptidase-T. 

Also known as: Ubiquitin Aldehyde (Ub-H)

A potent and highly specific inhibitor of all ubiquitin C-terminal hydrolases. This protein blocks the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.

Use & Storage

Use: 

Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit UCHs in vitro are 2-5 μM. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles. Avoid presence of amino-containing compounds. Do not lyophilize. Do not neutralize until immediately prior to use.

Literature

References: 

Hershko A. and Rose I.A. (1987) Proc.Natl.Acad. Sci. 84:1829-1833
Melandri F.D., et al. (1996) Biochem. 35:12893-12900
Dunten R.L. and Cohen, R.E. (1989) J.Biol.Chem. 264:1673916747