U-120

Material Data Sheet

FLAG (DYKDDDDK)-Ubiquitin

Price: $65.00
Catalog #: U-120

FLAG (DYKDDDDK)-Ubiquitin

Price: 
$65
Quantity: 
1 mg

This fully functional N-terminal FLAG-tagged ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The epitope tag is a hydrophilic octapeptide (DYKDDDDK) derived from the sequence of the bacteriophage 7 gene-10 product. This tag is specifically recognized by anti-FLAG antibodies M1, M2 or M5. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Product Information

MW: 
9.3 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 10 mg/ml
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions

Storage: 

Lyophilized powder at 4°C. Solubilized stock solution at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Coux O., et al. (1996) Ann.Rev.Biochem. 65: 801-847
Einhauer A. and Jungbaer A. (2001) J.Chromatog. 921:25-30
Einhauer A. and Jungbaer A. (2001) J.Biochem.Biophys.Meth. 49:455-465
Hopp, T., et al. (1988) Biotechnology. 6:1204-1210
Knappik A., et al. (1994) Biotechniques. 17:754-761
Prickett K., et al. (1989) Biotechniques. 7:580-589
Slootstra J.W., et al. (1997) Mol.Divers. 2:156-164
Witzgall R., et al. (1994) Anal.Biochem. 223:291-8