U-115

Material Data Sheet

myc-Ubiquitin

Price: $65.00
Catalog #: U-115

myc-Ubiquitin

Price: 
$65
Quantity: 
1 mg
Data Sheet: 

This fully functional N-terminal myc-tagged ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The tag sequence (EQKLISEEDL) is an epitope derived from the C-terminal region of the human proto-oncoprotein p62c-myc. This tag is specifically recognized by anti-myc antibodies or anti-myc-agarose. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteasome. Ubiquitination also affects proteasome-independent events such as protein localization, activity and function.

Product Information

MW: 
9.3 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 10 mg/ml
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions

Storage: 

Lyophilized powder at 4°C. Solubilized stock solution at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Coux O., et al. (1996) Ann.Rev.Biochem. 65: 801-847
Ellison M.J and Hochstrasser M. (1991) J.Biol.Chem. 266: 21150-21157
Evan G.I., et al. (1985) Mol.Cell.Biol. 5: 3610-3616
Fritze C.E. and Anderson T.R. (2000) Meth. Enz. 327: 3-16
Johnson E.S., et al. (1992) EMBO J. 11: 497-505
Hillman M.C.,et al. (2001) Prot.Exp.Purif. 23: 359-368
Hilpert K., et al. (2001) Prot.Engin. 14: 803-806
Hodgins R.R., et al. (1992) J.Biol.Chem. 267: 8807-8812
Terpe K (2003) Appl.Microbiol.Biotech. 60: 523-533