U-110

Material Data Sheet

HA-Ubiquitin

Price: $69.00
Catalog #: U-110

HA-Ubiquitin

Price: 
$69
Quantity: 
1 mg
Data Sheet: 

This fully functional N-terminal HA-tagged ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The HA peptide sequence (YPYDVPDYA) is an epitope derived from the influenza hemagglutinin protein. This tag is specifically recognized by anti-HA antibodies and anti-HA-agarose. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Product Information

Stock: 
2.5 mg/ml (250 µM) in 10 mM HEPES pH 7.5
MW: 
9.8 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical concentration to support in vitro conjugation is 5-50 µM depending on conditions 

Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles.