U-102

Material Data Sheet

Phospho-Ubiquitin (S65)

Price: $245.00
Catalog #: U-102

Phospho-Ubiquitin (S65)

Price: 
$245
Quantity: 
150 µg
Data Sheet: 

Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress.  PINK is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function.  In damaged (depolarized) mitochondria PINK becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Miofusins.  Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by PINK-mediated phosphorylation of PARK2 at serine 65, and PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65).  This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2.

Recombinant Phospho-Ubiquitin (pS65) is generated enzymatically using PINK1 kinase from Red Flour Beetle (Tribolium castaneum).

Product Information

Stock: 
X mg/ml (X µM) in 10 mM HEPES pH 7.5
MW: 
8.65 kDa
Purity: 
> 98% by SDS-PAGE, > 95% by AQUA analysis
Western Blot Data: 

Use & Storage

Use: 

Reaction conditions will need to be optimized for each specific application. 

Storage: 

Store at -20°C.  Avoid multiple freeze/thaw cycles.