U-100Sc

Material Data Sheet

Ubiquitin

Price: $59.00
Catalog #: U-100Sc

Ubiquitin

Price: 
$59
Quantity: 
5 mg
Data Sheet: 

Highly purified ubiquitin free of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic eukaryotic protein. It is covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP). The major role of ubiquitination is to target cellular proteins for the ATP-dependent degradation by the 26S proteasome and proteasome-independent or regulatory events such as protein localization, activity and function. This protein sequence is for S.cerevisiae (P61864) and is exactly the same for S.pombe.

Product Information

MW: 
8.6 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 20 mg/ml
Supplied: 
This is a lyophilized powder
Background: 
Protein Sequences:<strong><em>H.sapiens</em> Ub</strong>: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT LSDYNIQKESTLHLVLRLRGG<strong><em>S.cerevisiae</em> Ub:</strong> MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRT LSDYN IQKESTLHLVLRLRGG

Use & Storage

Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions 

Storage: 

Lyophilized powder at 4°C. Solubilized stock solution at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ciechanover A., et al. (1980) J.Biol.Chem. 255: 7525-7528
Coux O., et al. (1996) Ann.Rev.Biochem. 65: 801-847
Glickman M.H. and Ciechanover A. (2002) Physiol.Rev. 82:373-428
Hershko A. and Ciechanover A. (1992) Ann.Rev.Biochem. 61: 761-807
Schwartz A.L and Ciechanover A. (1999) Ann.Rev.Med. 50: 57-74.
Smalle J. and Vierstra. R.D. (2004) Ann.Rev.Plant.Biol. 55:555-590
Wilkinson K.D. and Audhya T.K. (1981) J.Biol.Chem. 256: 9235-9241