U-100Pf

Material Data Sheet

Ubiquitin

Catalog #: U-100Pf

Ubiquitin

Datasheet: 
application/pdf icon100Pf_parastic Ub.pdf
Product Source: 

P.falciparum

Highly purified ubiquitin free of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic eukaryotic protein. It is covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP). The major role of ubiquitination is to target cellular proteins for the ATP-dependent degradation by the 26S proteasome and proteasome-independent or regulatory events such as protein localization, activity and function. This protein sequence is for protozoan parasite P.falciparum (NP_701482) which causes malaria. Considering its conserved role among eukaryotes, this system is expected to regulate key molecular events driving the parasite life cycle, including parasite discrete apicomplexan mechanisms such as host cell invasion and apicoplast formation.

Product Information
Quantity: 

5 mg

Price: 

$50

MW: 

8.6 kDa

Purity: 

> 95% by SDS-PAGE

Solubility: 

Aqueous solutions up to 20 mg/ml

Supplied: 

This is a lyophilized powder

Background: 

Protein Sequences:

H.sapiens Ub: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT LSDYNIQKESTLHLVLRLRGG

P.falcipirum Ub: MQIFVKTLTGKTITLDVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG

Use & Storage
Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions

 

Storage: 

Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Ciechanover A., et al. (1980) J.Biol.Chem. 255: 7525-7528
Coux O., et al. (1996) Ann.Rev.Biochem. 65: 801-847
Glickman M.H. and Ciechanover A. (2002) Physiol.Rev. 82:373-428
Hershko A. and Ciechanover A. (1992) Ann.Rev.Biochem. 61: 761-807
Schwartz A.L and Ciechanover A. (1999) Ann.Rev.Med. 50: 57-74.
Smalle J. and Vierstra. R.D. (2004) Ann.Rev.Plant.Biol. 55:555-590
Wilkinson K.D. and Audhya T.K. (1981) J.Biol.Chem. 256: 9235-9241