U-100H

Material Data Sheet

Ubiquitin

Price: $89.00
Catalog #: U-100H

Ubiquitin

Price: 
$89
Quantity: 
10 mg
Data Sheet: 

Highly purified ubiquitin processed for the quantitative removal of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Product Information

MW: 
8.5 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 50 mg/ml
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions 

Storage: 

Lyophilized powder at 4°C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ciechanover A., et al. (1980) J.Biol.Chem. 255: 7525-7528
Coux O., et al. (1996) Ann.Rev.Biochem. 65: 801-847
Glickman M.H. and Ciechanover A. (2002) Physiol.Rev. 82:373-428
Hershko A. and Ciechanover A. (1992) Ann.Rev.Biochem. 61: 761-807
Schwartz A.L and Ciechanover A. (1999) Ann.Rev.Med. 50: 57-74.
Wilkinson K.D. and Audhya T.K. (1981) J.Biol.Chem. 256: 9235-9241