Material Data Sheet
Highly purified ubiquitin free of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic eukaryotic protein. It is covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP). The major role of ubiquitination is to target cellular proteins for the ATP-dependent degradation by the 26S proteasome and proteasome-independent or regulatory events such as protein localization, activity and function. This protein sequence is for A.thaliana (P59263) and is exactly the same for barley, oat, soya bean and other plants.
Purity:> 95% by SDS-PAGE
Solubility:Aqueous solutions up to 20mg/ml
Supplied:This is a lypphilized powder
Background:<em>H.sapiens</em> Ub: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT<br />LSDYNIQKESTLHLVLRLRGG<em>A.thaliana</em>Ub:MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN IQKESTLHLV LRLRGG
Use & Storage
Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions
Lyophilized powder at 4oC. Solubilized stock solution at -20oC. Avoid multiple freeze/thaw cycles.
Burke T.J., et al. (1988) Mol. Gen. Genet. 213:435-443
Ciechanover A., et al. (1980) J. Biol. Chem. 255: 7525-7528
Coux O., et al. (1996) Ann. Rev. Biochem. 65: 801-847
Glickman M.H. and Ciechanover A. (2002) Physiol. Rev. 82:373-428
Hershko A. and Ciechanover A. (1992) Ann. Rev. Biochem. 61: 761-807
Schwartz A.L and Ciechanover A. (1999) Ann. Rev. Med. 50: 57-74.
Smalle J. and Vierstra. R.D. (2004) Ann.Rev.Plant.Biol. 55:555-590
Wilkinson K.D. and Audhya T.K. (1981) J. Biol. Chem. 256: 9235-9241