U-100At

Material Data Sheet

Ubiquitin

Price: $65.00
Catalog #: U-100At

Ubiquitin

Price: 
$65
Quantity: 
5 mg
Data Sheet: 

Highly purified ubiquitin free of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic eukaryotic protein. It is covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteasome Pathway (UPP). The major role of ubiquitination is to target cellular proteins for the ATP-dependent degradation by the 26S proteasome and proteasome-independent or regulatory events such as protein localization, activity and function. This protein sequence is for A.thaliana (P59263) and is exactly the same for barley, oat, soya bean and other plants.

Product Information

MW: 
8.6 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 20mg/ml
Supplied: 
This is a lypphilized powder
Background: 
H.sapiens Ub: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG A.thaliana Ub:MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN IQKESTLHLV LRLRGG

Use & Storage

Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions

Storage: 

Lyophilized powder at 4°C. Solubilized stock solution at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Burke T.J., et al. (1988) Mol. Gen. Genet. 213:435-443
Ciechanover A., et al. (1980) J. Biol. Chem. 255: 7525-7528
Coux O., et al. (1996) Ann. Rev. Biochem. 65: 801-847
Glickman M.H. and Ciechanover A. (2002) Physiol. Rev. 82:373-428
Hershko A. and Ciechanover A. (1992) Ann. Rev. Biochem. 61: 761-807
Schwartz A.L and Ciechanover A. (1999) Ann. Rev. Med. 50: 57-74.
Smalle J. and Vierstra. R.D. (2004) Ann.Rev.Plant.Biol. 55:555-590
Wilkinson K.D. and Audhya T.K. (1981) J. Biol. Chem. 256: 9235-9241