U-100

Material Data Sheet

Ubiquitin

Catalog #: U-100

Ubiquitin

Datasheet: 
application/pdf iconU100_mUb.pdf
Product Source: 

mammalian

Highly purified ubiquitin processed for the quantitative removal of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Product Information
Quantity: 

10 mg

Price: 

$40

MW: 

8.5 kDa

Purity: 

> 95% by SDS-PAGE

Solubility: 

Aqueous solutions up to 50 mg/ml

Supplied: 

This is a lyophilized powder

Use & Storage
Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions

Storage: 

Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.

Literature
References: 

Ciechanover A., et al. (1980) J.Biol.Chem. 255: 7525-7528
Coux O., et al. (1996) Ann.Rev.Biochem. 65: 801-847
Glickman M.H. and Ciechanover A. (2002) Physiol.Rev. 82:373-428
Hershko A. and Ciechanover A. (1992) Ann.Rev.Biochem. 61: 761-807
Schwartz A.L and Ciechanover A. (1999) Ann.Rev.Med. 50: 57-74.
Wilkinson K.D. and Audhya T.K. (1981) J.Biol.Chem. 256: 9235-9241