SP-600

New
Material Data Sheet

BRD4 (49-460), FLAG-tag, His-tag

Price: $351.00
Catalog #: SP-600

BRD4 (49-460), FLAG-tag, His-tag

Price: 
$351
Quantity: 
100 µg
Data Sheet: 

Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory.  BRD4 has two N-terminal bromodomains and one NET (N-terminal Extra Terminal) domain.  BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA polymerase II.  BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.  This recombinant protein contains an N-terminal portion of BRD4 including both bromodomains and contains both His10 and FLAG tags at the N-terminus.  This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders.

Product Information

Stock: 
X  mg/ml (X mM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
52 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain
Storage: 

Store at -80°C