SP-490

New
Material Data Sheet

Miro1

Price: $195.00
Catalog #: SP-490

Miro1

Price: 
$195
Quantity: 
100 µg
Data Sheet: 

Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa.  Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules.  A number of publications have identified Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential part of the cellular machinery that participates in the removal of damaged mitochondria. 

Useful as an in vitro substrate for activated Parkin, this recombinant protein contains an N-terminal 6-His tag, and amino acids Ile186-Thr591 of NP_060777.3.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 2 mM TCEP
MW: 
50 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Reaction Conditions will be optimized for each specific application.  In a 50 μl reaction containing 1 μM activated Parkin E3 ligase (E3-160 or E3-162), 1 μM His6-Miro1 is fully converted to Ubiquitinated forms in 60 minutes.

Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles.