SP-485

New
Material Data Sheet

α-Synuclein

Price: $379.00
Catalog #: SP-485

α-Synuclein

Price: 
$379
Quantity: 
500 µg
Data Sheet: 

α-Synuclein is a member of a family of small, soluble proteins that also include β-, and γ-Synuclein.  α-Synuclein is predominantly expressed in neurons of the central nervous system, specifically in the presynaptic region of nerve terminals.  This protein cycles between a free, partially unfolded form and a helical, membrane-bound form.  α-Synuclein can aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates.  The fibrils and amyloidal forms are major components of Lewy Bodies and have been linked to the pathogenesis of Parkinson's disease dementia.  α-Synuclein aggregates can also be found associated with amyloid plaques in Alzheimer's disease.  This untagged recombinant protein may be used as an in vitro substrate for various Ubiquitin E3 ligases.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM DTT
MW: 
14 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Recombinant Human α-Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial α-Synuclein concentration of 0.5-2.5 μM.

Storage: 

Store at -80ºC.  Avoid freeze/thaw cycles