SP-452

Material Data Sheet

His6-FLAG-p53

Price: $255.00
Catalog #: SP-452

His6-FLAG-p53

Price: 
$255
Quantity: 
20 µg
Data Sheet: 

Tumor suppressor protein p53, a nuclear transcription factor, plays an essential role in the regulation of the cell cycle and is frequently mutated or inactivated in many cancers.  Numerous post-translational modifications modulate p53 activity including ubiquitination, phosphorylation, acetylation and methylation. The stability of p53 is regulated via the ubiquitin-proteasome pathway (UPP).  MDM2 is an oncogenic ubiquitin E3 ligase that ubiquitinates p53, inhibits its transcriptional activity and promotes its degradation.  Other E3 ligases that promote the proteasome-mediated degradation of p53 include Pirh2, COP1 and p300.  USP7 (HAUSP) stabilizes p53 by deubiquitination and induces p53-dependent cell growth repression and apoptosis.  Additional factors such as p14ARF and MdmX also modulate p53 function via the UPP.  This recombinant protein contains N-terminal 6-His and FLAG tags.

Product Information

Stock: 
X mg/ml (X mM) in 50 mM HEPES pH 7.5, 450 mM NaCl, 10% glycerol, 30 µM ZnCl2  Actual concentration varies with lot number.
MW: 
46 kDa
Purity: 
>75% by SDS-PAGE

Use & Storage

Use: 

Typical concentrations for in vitro assays are 0.5-2.5 mM depending on experimental conditions and detection method.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Brooks C.L. and Gu W. (2006) Mol.Cell. 21:307-15

Dornan D., et. al.  (2004) Nature 429:86-92

Grossman S.R., et. al. (2003) Science 300:342-344

Hu M., et. al.  (2006)  PLoS Biol. 4:228-239

Li M, et. al.   (2002) Nature 416:648-653

Li M, et. al.   (2003) Science 301:1972-1975

Meulmeester E. and Jochemsen A.G.  (2008) Curr.Canc.Drug Targets 8:87-97

Sheng Y., et. al.   (2006) Nat.Struc.Mol.Biol. 13:285-291