SP-450

Material Data Sheet

His6-p53

Price: $279.00
Catalog #: SP-450

His6-p53

Price: 
$279
Quantity: 
20 µg
Data Sheet: 

Tumor suppressor protein p53, a nuclear transcription factor, plays an essential role in the regulation of cell cycle and is frequently mutated or inactivated in many cancers. Numerous post-translational modifications modulate p53 activity including ubiquitination, phosphorylation, acetylation and methylation. The stability of p53 is regulated via the ubiquitin-proteasome pathway (UPP). MDM2 is an oncogenic ubiquitin E3 ligase that ubiquitinates p53, inhibits its transcriptional activity and promotes its degradation. Other E3 ligases that promote the proteasome-mediated degradation of p53 include Pirh2, COP1 and p300. USP7 (HAUSP) stabilizes p53 by deubiquitination and induces p53-dependent cell growth repression and apoptosis. Additional factors such as p14ARF and MdmX also modulate p53 function via the UPP. This recombinant protein is N-terminally tagged and produced in E.coli. Accession # NP_000537.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 450 mM NaCl, 10% glycerol. Actual concentration varies with lot number.
MW: 
45.6 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical concentrations for in vitro assays are 0.5-2.5 μM depending on experimental conditions and detection method. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.
Purity: 
≥ 96.2 % by HPLC.

Literature

References: 

Brooks CL and Gu W. (2006) Mol.Cell. 21:307-15
Dornan D, et al. (2004) Nature 429:86-92
Grossman S.R., et al. (2003) Science 300:342-344
Hu M., et al. (2006) PLoS Biol. 4:228-239
Kussie P.H., et al. (1996) Science 274:948-953
Lamb P. and Crawford L. (1986) Molec.Cell.Biol. 6:1379-1385, 1986.
Li M, et al. (2002) Nature 416:648-653
Li M, et al. (2003) Science 301:1972-1975
Meulmeester E. and Jochemsen A.G. (2008) Curr.Canc.Drug.Targets 8:87-97
Sheng Y, et al. (2006) Nat.Struc.Mol.Biol. 13:285-291