SP-300

Material Data Sheet

SUMO Conjugation Peptide Substrate, Biotin

Price: $109.00
Catalog #: SP-300

SUMO Conjugation Peptide Substrate, Biotin

Price: 
$109
Quantity: 
20 µg

This biotinylated peptide contains a Sumoylation consensus sequence and can be covalently conjugated to SUMO1, SUMO2 and SUMO3 proteins in the presence of the E1 activating (SAE1/SAE2) and Ubc9 (UBE2I) conjugating enzymes. The biotin group allows for convenient and sensitive detection with avidin-linked reagents.

SUMO modification of proteins occurs on lysine residues generally found within a short consensus sequence containing the ψΚXE motif. This motif consists of ψ which represents a large hydrophobic amino acid (isoleucine, leucine or valine), K is the lysine that becomes modified, X is any residue and E is glutamic acid. The glutamic acid is the most highly conserved position other than the lysine.

Product Information

MW: 
1.53 kDa, (11 residues in length)
Solubility: 
Soluble in aqueous buffer up to 5 mg/ml.
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Add stock to in vitro or in vivo assays at desired concentration. Recommended concentration range is 50-100 μM depending on conditions. 

Storage: 

Store stock solution at -20°C. Avoid freeze/thaw cycles.

Purity: 
≥ 96.2 % by HPLC.

Literature

References: 

Johnson E.S. (2004) Annu.Rev.Biochem. 73:355-382
Okuma T., et al. (1999) Biochem.Biophys.Res.Comm. 254:693-698
Rodriguez M.S., et al. (2001) J.Biol.Chem. 276:12654-12659
Saitoh H. and Hinchey J. (2000) J.Biol.Chem. 275:6252-6258
Sampson D.A. et al. (2001) J.Biol.Chem. 276:21664-21669
Tatham M.H., et al. (2001) J.Biol.Chem. 276:35368-35374