SP-205

Material Data Sheet

E2-25K/UBE2K/UbcH1 Biotin

Price: $159.00
Catalog #: SP-205

E2-25K/UBE2K/UbcH1 Biotin

Price: 
$159
Quantity: 
25 µg

This biotinylated protein can be used as a positive control for sumoylation assays. It is modified
by SUMO-1, SUMO-2 and SUMO-3 proteins and can be detected with avidin-linked reagents.
E2-25K is a class II ubiquitin-conjugating E2 enzyme identified as a target for sumoylation
during a screen for novel SUMO-1 targets. The SUMO modification of E2-25K has been
extensively characterized in vitro (Pichler et al.). This substrate is efficiently sumoylated by the
SUMO activating E1 (SAE1/SAE2 or Aos1/Uba2) and the Ubc9 E2 enzyme in the absence of an
E3 ligase. The E2-25K SUMO acceptor site is unique and does not conform to the yKXE
consensus site, being located at Lys14 and functioning only in the context of an a-helix.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration will vary with specific lot number.
MW: 
25 kDa

Use & Storage

Use: 

Typical concentration to use in SUMO conjugation experiments in vitro is 1-5 μM depending on conditions.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.
Purity: 
> 95% by SDS-PAGE

Literature

References: 

Chen Z.J., et al. (1991) J.Biol.Chem. 266:15698-15704
Haldeman M.T., et al. (1997) Biochem. 36:10526-10537
Kalchman M.A.., et al. (1996) J.Biol.Chem. 271:19385-19394
Mastrandrea L.D., et al. (1998) Biochem. 37:9784-9792
Pickart C.M., et al. (1992) J.Biol.Chem. 267:14418-14423
Pichler A. et al. (2005) Nat.Struc.Mol.Biol. 12:264-269
Piotrowski J., et al. (1997) J.Biol.Chem. 272:23712-23721
Song S., et al. (2003) Mol.Cell 12:553-563
Yao T. and Cohen R.E. (2000) J.Biol.Chem. 275:36862-36868