SP-200

Material Data Sheet

E2-25K/UBE2K/UbcH1

Price: $159.00
Catalog #: SP-200

E2-25K/UBE2K/UbcH1

Price: 
$159
Quantity: 
50 µg

This protein can be used as a positive control for SUMOylation assays. UBE2K (E2-25K) is modified by SUMO-1, SUMO-2 and SUMO-3 and can be detected on Western blots using anti-UBE2K (E2-25K) (A-603) or anti-SUMO (A-712) antibodies
UBE2K (E2-25K) is a class II ubiquitin-conjugating E2 enzyme identified as a target for SUMOylation during a screen for novel SUMO-1 targets. The SUMO modification of UBE2K (E2-25K) has been extensively characterized in vitro (Pichler et al.). This substrate is efficiently SUMOylated by the SUMO activating E1 (SAE1/SAE2 or Aos1/Uba2) and the Ubc9 E2 enzyme in the absence of an E3 ligase. The UBE2K (E2-25K) SUMO acceptor site is unique and does not conform to the ψKXE consensus site, being located at Lys14 and functioning only in the context of an α-helix.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration will vary with specific lot number.
MW: 
25 kDa

Use & Storage

Use: 

Typical concentration to use in SUMO conjugation experiments in vitro is 1-5 μM depending on conditions.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.
Purity: 
> 95% by SDS-PAGE

Literature

References: 

Chen Z.J., et al. (1991) J.Biol.Chem. 266:15698-15704
Haldeman M.T., et al. (1997) Biochem. 36:10526-10537
Kalchman M.A.., et al. (1996) J.Biol.Chem. 271:19385-19394
Mastrandrea L.D., et al. (1998) Biochem. 37:9784-9792
Pickart C.M., et al. (1992) J.Biol.Chem. 267:14418-14423
Pichler A. et al. (2005) Nat.Struc.Mol.Biol. 12:264-269
Piotrowski J., et al. (1997) J.Biol.Chem. 272:23712-23721
Song S., et al. (2003) Mol.Cell 12:553-563
Yao T. and Cohen R.E. (2000) J.Biol.Chem. 275:36862-36868