Material Data Sheet


Price: $159.00
Catalog #: SP-200


50 µg

This protein can be used as a positive control for SUMOylation assays. UBE2K (E2-25K) is modified by SUMO-1, SUMO-2 and SUMO-3 and can be detected on Western blots using anti-UBE2K (E2-25K) (A-603) or anti-SUMO (A-712) antibodies
UBE2K (E2-25K) is a class II ubiquitin-conjugating E2 enzyme identified as a target for SUMOylation during a screen for novel SUMO-1 targets. The SUMO modification of UBE2K (E2-25K) has been extensively characterized in vitro (Pichler et al.). This substrate is efficiently SUMOylated by the SUMO activating E1 (SAE1/SAE2 or Aos1/Uba2) and the Ubc9 E2 enzyme in the absence of an E3 ligase. The UBE2K (E2-25K) SUMO acceptor site is unique and does not conform to the ψKXE consensus site, being located at Lys14 and functioning only in the context of an α-helix.

Product Information

X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration will vary with specific lot number.
25 kDa

Use & Storage


Typical concentration to use in SUMO conjugation experiments in vitro is 1-5 μM depending on conditions.

Store at -80°C. Avoid multiple freeze/thaw cycles.
> 95% by SDS-PAGE



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