SP-100

Material Data Sheet

Biotin-Lysozyme Conjugation Substrate

Price: $159.00
Catalog #: SP-100

Biotin-Lysozyme Conjugation Substrate

Price: 
$159
Quantity: 
50 µg

Lysozyme has been used extensively as a substrate to study the Ubiquitin-dependent proteolytic pathway in vitro. This protein is an N-end rule substrate, since the recognition of protein requires the binding of an E3 ligase to the N-terminal lysine residue.  Ubiquitin-lysozyme conjugates can be formed in the presence of ATP, Ubiquitin and the appropriate conjugation enzyme source(s). This substrate protein can be used as a positive control with crude fractions (F-340, F-360, F-370, F-372) and conjugation kits (K-915, K-930, K-935, K-960).  Additionally, this lysozyme is labeled with biotin for use with avidin affinity/detection reagents.

Product Information

MW: 
14 kDa
Solubility: 
Soluble up to 1 mg/ml in sterile, deionized water
Supplied: 
Lyophilized from a solution in HEPES, pH 8.

Use & Storage

Use: 

Typical concentration as an in vitro conjugation substrate is 5 - 20 μM depending on conditions.

Storage: 

Store at -80°C once reconstituted.  Avoid multiple freeze/ thaw cycles.

Purity: 
>75% using reducing SDS-PAGE and visualized by Colloidal Coomassie Blue stain.

Literature

References: 

Hershko A., et al. (1980) Proc.Natl.Acad, Sci. 77:1783-1786
Hough R. and Reichsteiner M. et al. (1986) J.Biol.Chem. 261:2391-2399
Ganoth D., et al. (1988) J.Biol.Chem. 263:12412-12419
Gonda D.K., et al. (1989) J.Biol.Chem. 264:16700-16712
Reiss Y., et al. (1988) J.Biol.Chem. 263:2693-2698