Material Data Sheet
Fluorogenic tri-peptide substrate for measuring the "trypsin-like" peptidase activity of the 20S proteasome. Each of the two 20S internal β-type rings harbors three different proteolytically active sites: β1 ("post-glutamyl peptide hydrolase" site), β2 ("trypsin-like" site) and β5 ("chymotrypsin-like" site). This peptide is also substrate for the Kex2 endopeptidase from S. Cervisiae, which has substrate specificity toward the C-terminal side of LR, PR and RR sequences. In addition, the peptide can be cleaved by Kalikrein 5 with a P1 site preference for basic residues (R and K).
Purity:> 99% as adjudged by thin layer chromatography.
Solubility:Soluble in methanol up to 50 mg/ml. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.
Use & Storage
Add from stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)
Store lyophilized powder at -20°C for at least one year. Stock solution at -20°C for up to 1 month. Avoid multiple freeze/thaw cycles.
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