S-300

Material Data Sheet

Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)

Price: $89.00
Catalog #: S-300

Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)

Price: 
$89
Quantity: 
5 mg

Fluorogenic tri-peptide substrate for measuring the "trypsin-like" peptidase activity of the 20S proteasome. Each of the two 20S internal β-type rings harbors three different proteolytically active sites: β1 ("post-glutamyl peptide hydrolase" site), β2 ("trypsin-like" site) and β5 ("chymotrypsin-like" site). This peptide is also substrate for the Kex2 endopeptidase from S. Cervisiae, which has substrate specificity toward the C-terminal side of LR, PR and RR sequences. In addition, the peptide can be cleaved by Kalikrein 5 with a P1 site preference for basic residues (R and K).

Product Information

Structure: 
Purity: 
> 99% as adjudged by thin layer chromatography.
Solubility: 
Soluble in methanol up to 50 mg/ml. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.

Use & Storage

Use: 

Add from stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)

Storage: 
Store lyophilized powder at -20°C for at least one year. Stock solution at -20°C for up to 1 month. Avoid multiple freeze/thaw cycles.

Literature

References: 

Kessler B.M., et al. (2001) Chem.&Biol. 8 :913-929
Kisselev A.F., et al. (1999) Mol.Cell 4: 395-402
Meng L., et al. (1999) Proc.Natl.Acad.Sci. 96: 10403-10408
Michael I.P., et al. (2005) J.Biol.Chem. 96: 10403-10408
Mizuno K., et al. (1989) Biochem.Biophys.Res.Comm. 159: 305 –11
Reidilinger, J. et al. (1997). J. Biol. Chem. 272: 24899-905
Tamura T., et al. (1996) Science 274: 1385-1389