S-230

Material Data Sheet

Z-Leu-Leu-Glu-AMC 

Price: $135.00
Catalog #: S-230

Z-Leu-Leu-Glu-AMC 

Price: 
$135
Quantity: 
5 mg
Data Sheet: 

Fluorogenic substrate for measuring the peptidyl glutamyl peptide-hydrolyzing activity of the 20S proteasome. The 20S complex is composed of 28 subunits, arranged in an α7β7β7α7 stoichiometry. Each of the two internal -type rings harbors three different proteolytically active sites, provided by the amino-terminal residues of three constitutive subunits: β1 (post-glutamyl peptide hydrolase site), β2 (trypsin-like site) and β5 ( chymotrypsin-like site).

Product Information

Structure: 
Solubility: 
Soluble in DMSO. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.

Use & Storage

Use: 

Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)

Storage: 

Store DMSO stock at -20°C. Avoid multiple freeze/thaw cycles.

Purity: 
> 95% by TLC, HPLC. Structure confirmed by NMR.

Literature

References: 

Arendt C. S. and Hochstrasser M. (1997) Proc. Natl. Acad. Sci. 94:7156-7161
Coux O., et al. (1996) Ann. Rev. Biochem. 65:801–847
Dick T. P., et al. (1998) J. Biol. Chem. 273:25637-25646
Kisselev A. F. et al. (1999) Mol. Cell. 4: 395-402