S-220

Material Data Sheet

Z-Leu-Leu-Leu-AMC (Z-LLL-AMC)

Price: $129.00
Catalog #: S-220

Z-Leu-Leu-Leu-AMC (Z-LLL-AMC)

Price: 
$129
Quantity: 
5 mg
Data Sheet: 

Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome. The 20S complex is composed of twenty-eight subunits, arranged in an α7β7β7α7 stoichiometry. Each of the two internal β-type rings harbors three different proteolytically active sites, provided by the amino-terminal residues of three constitutive subunits: β1 (post-glutamyl peptide hydrolase site), β2 (trypsin-like site) and β5 (chymotrypsin-like site).

Product Information

Structure: 
Purity: 
>95% by HPLC
Solubility: 
Soluble in DMSO to 10 mM (or greater). For best results, pellet dry compound prior to reconstitution.

Use & Storage

Use: 

Z-LLL-AMC is a fluorogenic substrate for measuring the chymotrypsin-like activity of the 20S Proteasome Core.  Release of AMC fluorescence can be monitored with an excitation wavelength of 345 nm and an emission wavelength of 445 nm.  Reaction conditions will need to be optimized for each specific application.

Storage: 

Store DMSO stock at -20°C. Avoid multiple freeze/thaw cycles.

Purity: 
>95% by TLC, HPLC. Structure confirmed by NMR.

Literature

References: 

Arendt C. S. and Hochstrasser M. (1997) Proc. Natl. Acad. Sci. 94:7156-7161
Chen P. and Hochstrasser M. (1996) Cell 86:961-972
Coux O., et al. (1996) Ann. Rev. Biochem. 65:801–847
Dick T. P., et al. (1998) J. Biol. Chem. 273:25637-25646
Heinemeyer W., et al. (1997) J. Biol. Chem. 272:25200-25209
Kisselev A. F. et al. (1999) Mol. Cell. 4: 395-402