S-100

Material Data Sheet

Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)

Price: $119.00
Catalog #: S-100

Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)

Price: 
$119
Quantity: 
5 mg

Fluorgenic tetra-peptide substrate for ubiquitin C-terminal hydrolases (UCHs, eg. UCHL3) and Isopeptidase T. This low molecular weight peptide represents a minimal sequence of C-terminal residues of ubiquitin where hydrolysis occurs at the UbGly76-X bond. The catalytic efficiency for hydrolysis of this substrate by deconjugating enzymes is several thousand-fold less than a more native substrate (Ub-AMC, Cat # U-550) since remote interactions with Ub-containing substrates stabilize catalytic transition states.

Product Information

MW: 
11 kDa
Structure: 
Purity: 
> 95% by HPLC
Solubility: 
Soluble in DMSO. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.
Western Blot Data: 

Use & Storage

Use: 

Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)

Storage: 

Store lyophilized powder or stock solution at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dang L.C. et al. (1998) Biochemistry 37:1868 -1879
Mayer A.N. and Wilkinson K.D. (1989) Biochemistry 28:166-172
Stein R.L., et al. (1995) Biochemistry 34:12616 -12623
Wilkinson K.D., et al. (1995) Biochemistry 34:14535-14546
Wold W.S.M. and Tollefson A.E. (2008) Meth.Mol.Med. 131:251-255