Material Data Sheet

SUMO1 Conjugation Kit

Price: $355.00
Catalog #: K-710

SUMO1 Conjugation Kit

1 Kit
Data Sheet: 

This kit is designed for the conjugation of the Ubiquitin-like modifier SUMO1 to protein substrates in vitro, which requires the activities of the heterodimeric human E1 activating enzyme (SAE1/SAE2) and the Ubc9 E2 enzyme.  This kit does not include a SUMO E3 ligase, which may be needed for effective SUMOylation of some proteins.

NOTE: Kit contains reagents sufficient for 5-10 x 20 μl reactions.

Product Information

Western Blot Data: 
Western Blot Data: 
The Ubiquitin-like SUMO1 is conjugated to a variety of proteins in the presence of Ubc9 and the SUMO E1 activating enzyme (SAE1/SAE2 in human, or Aos1/Uba2p in yeast). The heterodimeric SAE1/SAE2 complex (38 and 70 kDa respectively) uses ATP to adenylate the C-terminal glycine residue of SUMO1, forming a thiolester bond with the SAE2 subunit. The second step is the trans-esterification reaction whereby the activated SUMO1 is transferred to Cys93 of Ubc9. Ubc9 is a member of the E2 family and is homologous to Ubiquitin conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it sometimes interacts directly with protein substrates that are modified by SUMOylation, and may play a role in substrate recognition. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis and protein function and stability.

Store at -80°C. Avoid multiple freeze/thaw cycles.