K-705

Material Data Sheet

His6-tagged SUMO Protein Set (1,2,3)

Price: $129.00
Catalog #: K-705

His6-tagged SUMO Protein Set (1,2,3)

Price: 
$129
Quantity: 
1 Kit

The ubiquitin-like SUMO-1, SUMO-2 and SUMO-3 proteins are conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. Human SUMO-1 shares 46% and 47% identity with SUMO-2 and SUMO-3 respectively. SUMO-1 is usually conjugated to proteins as a monomer. SUMO-2 shares 86% identity with SUMO-3, and these isoforms are known to form SUMO chains. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4, E2-25K). Unlike ubiquitination, SUMOylation does not appear to target proteins for degradation by the proteasome. SUMO modification has been implicated in diverse functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis and protein stability.

Product Information

Stock: 
50mM HEPES pH 8.0, 150mM NaCl, 1 mM DTT.
Purity: 
> 95 % by SDS-PAGE
Supplied: 
ProteinMWConcentrationQuantityHis6-SUMO-111.95 kDaX mg/ml (X μM)100 μgHis6-SUMO-211.35 kDaX mg/ml (X μM)100 μgHis6-SUMO-311.45 kDaX mg/ml (X μM)100 μg

Use & Storage

Use: 

Typical concentration to support conjugation reaction in vitro is 10 μM-50 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Adams M. D., et al. (1993) Nat.Genet. 4: 373-380
Dai K.-S. and Liew C.-C. (2001) J.Biol.Chem. 276: 23992-23999
Desterro J.M., et al. (1997) FEBs. Lett. 417:297-300
Dohmen R.J. (2004) Biophys. Biochem. Acta. 1695:113-131
Chung T.L., et al. (2004) J.Biol.Chem. 279: 39653-39662.
Gill G. (2004) Genes.Dev. 18:2046-2059
Hilgarth R.S., et al. (2004) J.Biol.Chem. 279: 53899-53902
Huang W-C.et al. (2004) Eur. J. Biochem. 271: 4114-4122
Kamitani T., et al. (1998) J.Biol.Chem. 273: 11349-11353
Lapenta V. et al. (1997) Genomics 40: 362-366
Mannen H., et al. (1996) Biochem.Biophys.Res.Comm. 222:178-180
Meluh P.B. and Koshland D. (1995) Mol. Biol. Cell 6: 793-807
Okama T., et al. (1999) Biochem. Biophys. Res. Comm. 254:693-698
Rodriguez M.S et al. (2001) J. Biol. Chem. 276:12654-59
Saitoh H. and Hinchey J. (2000) J.Biol. Chem. 275:6252-6258
Seeler J-S. and Dejean A. (2003) Nat. Rev. 4:690-699
Su H-L., et al. (2002) Gene 296:65-73
Subramanian L., et al. (2003) J.Biol. Chem. 278:9134-9141
Schwartz D.C. and Hochstrasser M. (2003) Tren. Biochem.Sci. 28:321-328
Tatham M.H., et al. (2001) J. Biol. Chem. 276:35368-35374