K-280

Material Data Sheet

CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate

Price: $455.00
Catalog #: K-280

CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate

Price: 
$455
Quantity: 
1 Kit
Data Sheet: 

CHIP (Carboxy terminus of HSP70-Interacting Protein) is a U-Box ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of misfolded chaperone substrates. CHIP functions in coordination with several chaperone complexes, including HSP40, HSP70, and HSP90.  CHIP activity may be modulated by the deubuiquitinase Ataxin-3, which restricts the length of ubiquitin chains attached to CHIP substrates and prevents further chain extension.  This kit is designed for in vitro ubiquitination of user-supplied substrates by CHIP or the CHIP/HSP70/HSP40 ternary complex.  Ubiquitinated proteins can be used in downstream applications, or analyzed by Western blot using antibodies specific for the target protein.  A control substrate and detection antibody are included in the kit.

NOTE:  Kit contains reagents sufficient for 10 x 30 μl reactions and 5 Western Blots (mini-gel format).

Storage: 

Store protein components at -80oC. Avoid multiple freeze/thaw cycles. Loading Buffer may be stored at room temperature. Mg2+-ATP and α-Glow Fold™ antibody may be stored at -20oC.

Literature

References: 

Connell P., et al.  (2001)   Nat. Cell Bio.  3: 93-96

Qian S.B., et al.   (2006)   Nature   440:  551-555

Scaglione K.M., et al. (2011)   Mol. Cell  43: 599-612