K-260

Material Data Sheet

cIAP-1/HIAP2 Ubiquitin Ligase Kit

Price: $355.00
Catalog #: K-260

cIAP-1/HIAP2 Ubiquitin Ligase Kit

Price: 
$355
Quantity: 
1 Kit
Data Sheet: 

Cellular inhibitor of apoptosis protein 1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases.  Structurally, cIAP-1 is comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD).  Functionally, cIAP-1 inhibits caspases through the direct interaction of its BIR domain with the active caspase.  The ring finger domain of cIAP-1 also functions as an E3 ubiquitin ligase to ubiquitinate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain.  This kit is designed for in vitro cIAP-mediated ubiquitination of user-supplied substrates.  Ubiquitinated proteins can be used in downstream applications, or analyzed by Western blot using antibodies specific for the target protein.  A detection antibody (AF8181) is included in the kit to monitor autoubiquitination of cIAP-1.

NOTE:  Kit contains reagents sufficient for 10 x 30 μl reactions and 5 Western Blots (mini-gel format).

Literature

References: 

Gyrd-Hansen M. & Meier P.  (2010) Nat. Rev. Cancer 10: 561-574

Bertrand M.J, et al. (2011) PLoS ONE  6: E22356-E22356

Kulathila R., et al. (2009) Acta Crystallogr. D 65: 58-66

Lopez J., et al. (2011) Mol. Cell 42: 569-583