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Mdm2/HDM2 Ubiquitin Ligase Kit - S5a Substrate
Mdm2/HDM2 Ubiquitin Ligase Kit - S5a Substrate
The RING-finger ubiquitin E3 ligase Mdm2/HDM2 (Murine Double Minute 2) is an important regulator of the tumor suppressor protein p53. Mdm2/HDM2 ligase activity is at least partially responsible for the ubiquitination and subsequent proteasomal degradation of p53 and other proteins. This kit is designed for in vitro Mdm2/HDM2-mediated ubiquitination of user-supplied substrates such as p53 (SP-450), β-Arrestin, and DYRK2. Ubiquitinated proteins can be used in downstream applications, or analyzed by Western blot using antibodies specific for the target protein. A control substrate, S5a (SP-400) and detection antibody (AF5540) are included in the kit.
Store protein components at -80oC. Avoid multiple freeze/thaw cycles. Loading Buffer may be stored at room temperature. Mg2+-ATP and α-S5a antibody may be stored at -20oC.
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