K-102

Material Data Sheet

MuRF1/TRIM63 Ubiquitin Ligase Kit - S5a Substrate

Price: $395.00
Catalog #: K-102

MuRF1/TRIM63 Ubiquitin Ligase Kit - S5a Substrate

Price: 
$395
Quantity: 
1 Kit
Data Sheet: 

MuRF1 (Muscle-specific RING-finger protein 1) is a RING-finger E3 ligase found in striated muscle (heart and skeletal) and iris tissues. The protein contains both RING and B-box zinc fingers, and a coiled-coil tripartite fold known as TRIM. MuRF1 ligase activity regulates the proteasomal degradation of cardiac troponin 1 and other sarcomeric-associated proteins. This kit is designed for in vitro MuRF1-mediated ubiquitination of user-supplied substrates such as cardiac troponin 1, MYH7, or muscle creatine kinase. Ubiquitinated proteins can be used in downstream applications, or analyzed by Western blot using antibodies specific for the target protein. A control substrate, S5a (SP-400) and detection antibody (AF5540) are included in the kit.

Storage: 

Store protein components at -80oC. Avoid multiple freeze/thaw cycles. Loading Buffer may be stored at room temperature. Mg2+-ATP and α-S5a antibody may be stored at -20oC.

Literature

References: 

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Kedar V., et al. (2004) Proc.Natl.Acad.Sci. 101: 18135-18140

McElhinny A.S., et al. (2002) J.Cell.Biol. 157: 125-136

Mrosek M., et al. (2007) FASEB J. 21: 1383-1392

Pizon V., et al. (2002) J.Cell.Sci. 115: 4496-4482

Reid M.B. (2005) Am.J.Physiol.Regul.Integ.Comp.Physiol. 288: 1423-1431

Wistow G., et al. (2002) Mol.Vis. 8: 185-195

Witt S.H., et al. (2005) J.Mol.Biol. 340: 713-722

Zhao, T-J., et al. (2007) J. Biol. Chem. 282: 12022-12029