I-502

Material Data Sheet

Nedd8 - E1 Enzyme (NAE Inhibitor)

Price: $125.00
Catalog #: I-502

Nedd8 - E1 Enzyme (NAE Inhibitor)

Price: 
$125
Quantity: 
1 mg

NAE Inhibitor contains the active component of MLN4924, a cell-permeable, small molecule inhibitor of Nedd8 activating enzyme (NAE).  By inhibiting the Neddylation pathway this molecule disrupts cullin-RING ligase activity, leading to inhibition of proliferation or apoptotic death of various human tumor cells through the deregulation of S-phase DNA synthesis.  Mutations in UBA3, a gene encoding one of the subunits of Nedd8 activating enzyme, have been reported to cause resistance to MLN4924 in some tumor cell lines.

Product Information

FW: 
444
Purity: 
>99% by HPLC
Solubility: 
Provided as a 20 mM stock solution in DMSO
Activity: 

Depending on cell line, typical IC50 values in 72 hour assay are ≤ 1 µM.

Storage: 

Store lyophilized material at -20°C. Store reconstituted material at -20°C for up to 3 months. Avoid multiple freeze/thaw cycles.

Literature

References: 

Lin, J.J., et al. (2010) Cancer Res. 70: 10310-10320

Soucy, T.A., et al. (2009) Nature 458: 732-737