I-444

Material Data Sheet

Apcin

Price: $155.00
Catalog #: I-444

Apcin

Price: 
$155
Quantity: 
5 mg vial
Data Sheet: 

Apcin (Anaphase Promoting Complex Inhibitor) is a cell-permeable compound capable of slowing APC-dependent proteolysis.  Apcin functions by inhibiting substrate binding to Cdc20, the mitotic activator of the ubiquitin ligase activity of the anaphase-promoting complex/cyclosome (APC/C).  The mechanism of action is distinct from that of another APC/C inhibitor, proTAME (I-440), which binds APC/C and inhibits both Cdc20- and Cdh1-dependent proteolysis.  When used alone in cultured cells, apcin blocks mitotic exit only modestly. (concentrations of 50-100 µM are required to slow mitotic exit).  However, a strong synergistic effect is observed when apcin and proTAME are used simultaneously.  Co-administration of the compounds provides a more robust mitotic arrest than would be predicted by additive models.  This may be very beneficial when treating cells that do not efficiently convert proTAME to its active parent compound (TAME, tosyl-L-arginine methyl ester), or if experiments demand strong APC/C inhibition. 

Product Information

Stock: 
Lyophilized powder
FW: 
438.65
Structure (deprecated): 

C13H14Cl3N7O4     

Purity: 
> 95% by LC/MS and NMR data
Solubility: 
Soluble in DMSO to ≥ 20 mM

Use & Storage

Use: 

Optimal dose for each cell line needs to be established empirically, however we do not recommend the use of apcin as a single agent in most experimental designs.  HeLa and hTERT-RPE1 cells are strongly arrested in mitosis when treated with a combination of proTAME (3-12 µM) and apcin (25-50 µM).

Storage: 

Store at -20°C.   Avoid multiple freeze/thaw cycles.

Purity: 
> 95% by LC/MS and NMR data

Literature

References: 

 

Sackton K.L., et al. (2014)  Nature  doi:10.1038/nature13660

Zeng X., et al.  (2010)  Cancer Cell  18: 382-395

Zeng X. and King R.W.  (2012) Nature Chemical Biology 8: 383-392