When diluting proTAME into the cell growth medium, it is best to add the DMSO stock solution to an empty tube first, and then add a large volume of growth medium followed by immediate mixing.  proTAME is sparingly soluble in aqueous solutions.  To avoid precipitation, instant and highly effective mixing is necessary.  Optimal dose for each cell line needs to be established empirically due to cell-line specific differences in prodrug activation.  HeLa and hTERT-RPE1 cells activate the prodrug efficiently, with mitotic arrest observed at 12 µM compound.  MCF10a cells activate the prodrug much less efficiently.