I-197

Material Data Sheet

b-AP15 (VLX1500)

Price: $55.00
Catalog #: I-197

b-AP15 (VLX1500)

Price: 
$55 $375
Quantity: 
200 μg  1 mg
Data Sheet: 

Potent, reversible, cell-permeable inhibitor that specifically inhibits the 19S regulatory particle- associated deubiquitinases. b-AP15 has been shown to inhibit tumor progression in at least four solid tumor models, and also inhibits organ infiltration in an acute myeloid leukemia (AML) model. b-AP15 is made available through collaboration with Vivolux AB, Uppsala, Sweden. 

Product Information

FW: 
419
Purity: 
> 98% by HPLC. Structure confirmed by 1H-NMR.
Solubility: 
Supplied as 10mM stock in DMSO (4.19 mg/ml). May hydrolyze slowly in aqueous buffers.
Activity: 

Inhibits purified 19S deubiquitinase activity using Ub-AMC as substrate (in vitro IC50=2.1μM). 

Decreases HCT-116 cell viability at 72 hours (EC50=0.4μM).

Storage: 
Store at 2-8°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

D' Arcy, P., et al. (2011) Nature Medicine. 17:1636-1640

Cain, C. SciBX 4(47); doi:10.1038/scibx.2011.1313