I-160

Material Data Sheet

Ac-Leu-Leu-Nle-CHO (MG-101)

Price: $40.00

Catalog #: I-160

Ac-Leu-Leu-Nle-CHO (MG-101)

Price:

$40

Quantity:

5 mg

Data Sheet:

160_MG101.pdf

Structure:

Purity:

> 95% by TLC, HPLC. Structure confirmed by NMR.

Solubility:

Pellet dry compound prior to DMSO addition. Soluble up to 15 mg/ml.

Peptide aldehydes form a covalent hemiacetal adduct with the 20S proteasome, inhibiting the chymotrypsin-like peptidase activity. These inhibitors are reversible and cell-permeable, and also inhibit the activity of calpains and cathepsins.

Use & Storage

Use:

Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Pre-incubation with compound prior to assay is recommended for maximal effect. 20S proteasome Ki = 2 μM

Storage:

Store DMSO stock at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References:

Griscavage, J.M., et al. (1995) Biochem. Biophys. Res. Comm. 215:721729
Kisselev A.F., et al. (2003) J. Biol. Chem. 278:35869-35877
Myung J., et al. (2001) Med. Res. Rev. 4:245-273
Vinitsky A., et al. (1994) J. Biol. Chem. 269:29860-29860